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Nuclear localization of cancer stem cell marker CD133 in triple-negative breast cancer: a case report

Abstract

Aim and background. It has been recently demonstrated that the detection of stem cell niches in triple-negative (TN) breast cancer may provide good prognostic clues for this tumor.
Methods and study design.We investigated the subcellular expression and localization of the cancer stem cell marker CD133 in a TN breast cancer biopsy from a 42-year-old Caucasian woman with a histological diagnosis of high-grade invasive ductal breast carcinoma by immunohistochemistry, flow cytometry and quantitative real-time PCR (qRT-PCR).
Results.We describe for the first time in a TN breast cancer the nuclear mislocalization of CD133, which normally shows membrane localization and more sporadically cytoplasmic localization. We also found this aberrant expression with qRT-PCR analysis but not flow cytometry.
Conclusions. Nuclear localization of CD133 may be an indicator of poor prognosis in TN breast cancer, as it is known that surface molecules, when moving into the nucleus, can act as transcriptional regulators by interfering with molecular pathways directly connected to the proliferation and differentiation of tumor cells.

Tumori 2013; 99(5): e245 - e250

Article Type: CASE REPORT

DOI:10.1700/1377.15325

Authors

Monica Cantile, Francesca Collina, Massimiliano D’Aiuto, Massimo Rinaldo, Giuseppe Pirozzi, Carmela Borsellino, Renato Franco, Gerardo Botti, Maurizio Di Bonito

Article History

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Authors

  • Cantile, Monica [PubMed] [Google Scholar]
    Pathology Unit, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • Collina, Francesca [PubMed] [Google Scholar]
    Pathology Unit, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • D’Aiuto, Massimiliano [PubMed] [Google Scholar]
    Department of Breast Surgery and Oncology, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • Rinaldo, Massimo [PubMed] [Google Scholar]
    Department of Breast Surgery and Oncology, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • Pirozzi, Giuseppe [PubMed] [Google Scholar]
    Pathology Unit, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • Borsellino, Carmela [PubMed] [Google Scholar]
    Centro Polispecialistico “Check Up”, Salerno, Italy
  • Franco, Renato [PubMed] [Google Scholar]
    Pathology Unit, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • Botti, Gerardo [PubMed] [Google Scholar]
    Pathology Unit, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy
  • Di Bonito, Maurizio [PubMed] [Google Scholar]
    Pathology Unit, Division of Breast Surgery, National Cancer Institute, Pascale Foundation, Naples, Italy

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