Long non-coding RNAs (lncRNAs), a class of regulatory RNAs, play a major role in various cellular processes. Long intergenic non-coding RNAs (lincRNAs), a subclass of lncRNAs, are involved in the trans- and cis-regulation of gene expression. In the case of cis-regulation, by recruiting chromatin-modifying complexes, lincRNAs influence adjacent gene expression.
We used quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) to evaluate the coexpression of LOC100287225, a lincRNA, and DCC, one of its adjacent genes that is often decreased in colorectal cancer, in pairs of tumor and adjacent tumor-free tissues of 30 colorectal cancer patients.
The qRT-PCR results revealed the misregulation of these genes during tumorigenesis. Their relative expression levels were significantly lower in tumor tissues than adjacent tumor-free tissues. However, the analysis found no significant correlation between reduced expression of these genes.
Our study demonstrated the concurrent misregulation of DCC and LOC100287225 in colorectal cancer.
Tumori 2017; 103(1): 40 - 43
Article Type: ORIGINAL RESEARCH ARTICLE
AuthorsMina Kazemzadeh, Reza Safaralizadeh, Mohammad Ali HosseinPour feizi, Mohammad Hossein Somi, Behrooz Shokoohi
- • Accepted on 07/08/2015
- • Available online on 30/09/2015
- • Published in print on 21/01/2017
This article is available as full text PDF.
- Kazemzadeh, Mina [PubMed] [Google Scholar] 1
- Safaralizadeh, Reza [PubMed] [Google Scholar] 1, * Corresponding Author (email@example.com)
- feizi, Mohammad Ali HosseinPour [PubMed] [Google Scholar] 1
- Somi, Mohammad Hossein [PubMed] [Google Scholar] 2
- Shokoohi, Behrooz [PubMed] [Google Scholar] 3
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz - Iran
Liver and Gastroenterology Diseases Research Center, Tabriz University of Medical Sciences, Tabriz - Iran
Department of Pathology, Tabriz University of Medical Sciences, Tabriz - Iran