Bladder carcinoma (BC) is one of the most common malignant cancers worldwide. Several genes related to the mechanism of BC have been studied in recent years, but the current understanding of BC is still rather limited. This study aimed to find new differentially expressed genes (DEGs) associated with the occurrence and development of BC.
In this work, we downloaded gene expression data from Gene Expression Omnibus under accession number GSE27448, which included 10 GeneChips from urinary BC tissues and 5 from normal tissues. DEGs were identified by the LIMMA package in R. Then the protein–protein interactions (PPIs) networks were analyzed with the database of Search Tool for the Retrieval of Interacting Genes, and gene ontology (GO) was applied to explore the underlying function of the DEGs using the Database for Annotation, Visualization and Integrated Discovery.
A total of 2,068 DEGs were found between BC and normal tissues. These genes were involved in 49 functional clusters. The top 10 highest degree nodes, such as POLR2F/2H (DNA directed RNA polymerase II polypeptide F/polypeptide H) and RPS14/15 (ribosomal protein S14/S15), were proven to be hub nodes in the PPIs network.
Genes such as
Tumori 2015; 101(1): 117 - 122
Article Type: ORIGINAL RESEARCH ARTICLE
AuthorsZhuomin Jia, Xing Ai, Fengling Sun, Tong Zang, Yawei Guan, Feng Gao
- • Accepted on 04/06/2014
- • Available online on 06/02/2015
- • Published in print on 20/03/2015
This article is available as full text PDF.
- Jia, Zhuomin [PubMed] [Google Scholar]
- Ai, Xing [PubMed] [Google Scholar] , * Corresponding Author (firstname.lastname@example.org)
- Sun, Fengling [PubMed] [Google Scholar]
- Zang, Tong [PubMed] [Google Scholar]
- Guan, Yawei [PubMed] [Google Scholar]
- Gao, Feng [PubMed] [Google Scholar]
Department of Urology, Military General Hospital of Beijing PLA, Beijing - PR China
Z. Jia and X. Ai contributed equally to this work and should be considered co–first authors