Fenofibrate is a specific agonist of PPARα, and is characterized by relatively low systemic toxicity. Recent studies have revealed that fenofibrate suppresses the growth of several cancer lines
The Eca-109 and TE1 cell lines were tested by the CCK-8 assay for cell proliferation. The multitarget click model was used to delineate the survival curve and radiosensitivity was determined after cells were treated with fenofibrate and/or x-ray radiation. Flow cytometry was used to examine the effect of fenofibrate and radiation on the cell cycle. The expression of vascular endothelial growth factor (VEGF) protein was detected by Western blot analysis.
When given alone, fenofibrate had a time- and concentration-dependent cytotoxic effect on cells. The dose-enhancement ratio for combined fenofibrate and radiation increased markedly compared with fenofibrate alone. Further, the ratio of cells in the G2/M phase after fenofibrate and radiation was higher than that after fenofibrate or irradiation alone. The expression of VEGF protein was suppressed after treatment with fenofibrate alone or fenofibrate plus radiation.
Fenofibrate can enhance the radiosensitivity of human esophageal carcinoma cells by increasing G2/M phase arrest. Modulation of VEGF expression could contribute
Tumori 2015; 101(1): 123 - 130
Article Type: ORIGINAL RESEARCH ARTICLE
AuthorsXiao-Qing Li, Jun-Dong Zhou, Shi-Tao Zou, Jian Yu, Xin-Jun Meng, Jin-Chang Wu
- • Accepted on 03/10/2014
- • Available online on 24/02/2015
- • Published in print on 20/03/2015
This article is available as full text PDF.
- Li, Xiao-Qing [PubMed] [Google Scholar] 1
- Zhou, Jun-Dong [PubMed] [Google Scholar] 1, 2
- Zou, Shi-Tao [PubMed] [Google Scholar] 1
- Yu, Jian [PubMed] [Google Scholar] 1
- Meng, Xin-Jun [PubMed] [Google Scholar] 1
- Wu, Jin-Chang [PubMed] [Google Scholar] 2, * Corresponding Author (firstname.lastname@example.org)
The Core Laboratory of Suzhou Cancer Center, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou - China
Department of Radiotherapy, Nanjing Medical University Affiliated Suzhou Hospital, Suzhou - China
Xiao-Qing Li and Jun-Dong Zhou contributed equally to this work