Advertisement

Correlation between deregulated expression of PER2 gene and degree of glioma malignancy

Abstract

Aims and background. Growing evidence indicates that disruption of circadian rhythms may be a risk factor for the development of glioma. However, the molecular mechanisms underlying the genetic regulation of circadian rhythms in glioma cells have yet to be explored.
Methods and study design. Using immunohistochemical staining and methylationspecific PCR techniques, we examined the expression of the period 2 (PER2) gene, one of the most important clock genes, epidermal growth factor receptor (EGFR), and proliferating cell nuclear antigen (PCNA) in 92 gliomas.
Results. Our results revealed disturbances in the expression of PER2 in most (52.17%) glioma cells compared with the expression in nearby noncancerous cells, and indicated that PER2 gene deregulation most likely occurs via methylation of PER2 promoters. The protein expression of PCNA and EGFR was significantly higher in highgrade than low-grade gliomas (P >0.05). Furthermore, a negative correlation was detected between the protein expression of PER2 and PCNA and EGFR in glioma.
Conclusions. Because the circadian clock regulates the expression of cell cycle-related genes, we suggest that disturbances in PER2 gene expression may disrupt the regulation of the circadian clock, thus enhancing the survival of cancer cells and promoting carcinogenesis.

Tumori 2014; 100(6): e266 - e272

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.1700/1778.19292

Authors

Fan Wang, Yong Luo, Caiyan Li, lvan Chen

Article History

This article is available as full text PDF.

  • If you are a Subscriber, please log in now.

  • Article price: Eur 36,00
  • You will be granted access to the article for 72 hours and you will be able to download any format (PDF or ePUB). The article will be available in your login area under "My PayPerView". You will need to register a new account (unless you already own an account with this journal), and you will be guided through our online shop. Online purchases are paid by Credit Card through PayPal.
  • If you are not a Subscriber you may:
  • Subscribe to this journal
  • Unlimited access to all our archives, 24 hour a day, every day of the week.

Authors

  • Wang, Fan [PubMed] [Google Scholar]
    Department of Neurosurgery, The First People’s Hospital of Jingmen, Jingmen, China
  • Luo, Yong [PubMed] [Google Scholar]
    Department of Neurosurgery, The First People’s Hospital of Jingmen, Jingmen, China
  • Li, Caiyan [PubMed] [Google Scholar]
    Department of Neurosurgery, The Second People’s Hospital of Jingmen, Jingmen, China
  • Chen, lvan [PubMed] [Google Scholar]
    Department of Neurosurgery, The First People’s Hospital of Jingmen, Jingmen, China

Article usage statistics

The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.

No supplementary material is available for this article.