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Clinico-pathological study of K-ras mutations in colorectal tumors in Saudi Arabia

Abstract

Aims and background. K-ras gene mutations contribute to the pathogenesis of colorectal cancer. We characterized K-ras mutations in colorectal tumors in patients in the Kingdom of Saudi Arabia, in terms of geographic area, age, gender, histology, stage, and anatomical localization.
Methods. Medical records and paraffin-embedded tumor samples from 150 consecutive patients with histologically proven colorectal adenocarcinoma referred to two centers in Saudi Arabia were analyzed using an LCD-array kit.
Results. K-ras mutations occurred in 56% of the patients; 48.7% of the mutations were in codon 12, most commonly p.G12V and p.G12D (each 35.6% of codon 12 mutations). Codon 13 mutations occurred in 7.3% of tumors: of these, most were p.G13D (90.9%) with the remainder p.G13R (9.1%). Codon 12 mutations overall were associated significantly with stage IVb tumors (P = 0.022) and rectal tumors (P = 0.028), with a trend of an association with a sigmoid location (P = 0.054). The p.G12V mutation was significantly associated with sigmoid tumors (P = 0.021) and negatively associated with left-sided tumors (P = 0.011), with a trend of an association with age ≥70 years (P = 0.062) and rectal tumors (P = 0.063). Other clinicopathological features were not significantly associated with K-ras mutations.
Conclusions. K-ras mutations are common among the Saudi colorectal cancer population, especially pG12V and pG12D in codon 12, and are more frequent in sigmoid and rectal adenocarcinomas and stage IVB tumors.

Tumori 2014; 100(1): 75 - 79

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.1700/1430.15819

Authors

Ali Zahrani, Magdy Kandil, Talha Badar, Mahmoud Abdelsalam, Abdulla Al-Faiar, Abdelsalam Ismail

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Authors

  • Zahrani, Ali [PubMed] [Google Scholar]
    Department of Oncology, Prince Sultan Military Medical City, Oncology Department, Riyadh, Saudi Arabia
  • Kandil, Magdy [PubMed] [Google Scholar]
    Department of Oncology, Prince Sultan Military Medical City, Oncology Department, Riyadh, Saudi Arabia
  • Badar, Talha [PubMed] [Google Scholar]
    Department of Oncology, Medical Oncology, Comprehensive Cancer Centre, King Fahad Medical City, Riyadh, Saudi Arabia
  • Abdelsalam, Mahmoud [PubMed] [Google Scholar]
    Department of Oncology, Prince Sultan Military Medical City, Oncology Department, Riyadh, Saudi Arabia
  • Al-Faiar, Abdulla [PubMed] [Google Scholar]
    Department of Pathology, Prince Sultan Military Medical City, Oncology Department, Riyadh, Saudi Arabia
  • Ismail, Abdelsalam [PubMed] [Google Scholar]
    Department of Oncology, Medical Oncology, Comprehensive Cancer Centre, King Fahad Medical City, Riyadh, Saudi Arabia

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