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TAp73 and ΔNp73 relative expression in Egyptian patients with lymphoid neoplasms

Abstract

Background and aims

The p73 gene has different isoforms with opposing anti- and pro-apoptotic functions. The pro-apoptotic activities are inhibited by overexpression of the dominant ΔNp73 isoform. The aim of this study was to detect the expression of the TAp73 and ΔNp73 isoforms in Egyptian patients with malignant lymphoid neoplasms. Their expression was analyzed by quantitative RT-PCR.

Patients and methods

The study included 30 B-NHL patients, 24 T-NHL patients, 16 ALL patients, 18 CLL patients, 22 patients with reactive lymphoid hyperplasia, and 6 healthy control subjects.

Results

ALL and CLL patients expressed both isoforms at higher levels compared to lymphoma patients. Higher expression of TAp73 was found in both B-NHL and T-NHL (around 4-fold and 16-fold, respectively) in comparison to ΔNp73 (2-fold and 14-fold, respectively). In CLL patients both isoforms showed higher expression levels in comparison to normal peripheral blood lymphocytes controls: nearly 27-fold for TAp73 and 233-fold for ΔNp73. All ALL patients showed higher expression of both studied isoforms than controls (9-fold for TAp73 and 386-fold for ΔNp73). The highest ΔNp73 expression along with a higher ΔNp73/TAp73 ratio (67-fold) was found in ALL patients compared with CLL patients (21-fold).

Conclusions

A considerable number of lymphoma patients lacked the expression of either or both isoforms, while all lymphoid leukemia patients expressed both isoforms. The expression pattern differences of p73 isoforms may reflect differences in the biology of these malignancies.

Tumori 2017; 103(3): 268 - 271

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/tj.5000506

Authors

Mona Salah Al-Din Hamdy, Zainab Ali El-Saadany, Manal Mohamed Makhlouf, Asmaa Ibrahim Salama, Nihal Salah Ibrahim, Alaa Amr Gad

Article History

Disclosures

Financial support: This work was partially funded by the Faculty of Medicine, Cairo University, Cairo, Egypt.
Conflict of interest: The authors state that they have no conflict of interest related to this paper.

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Authors

Affiliations

  • Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo - Egypt
  • Department of Pathology, National Cancer Institute, Cairo University, Cairo - Egypt

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