miRNA-650 exerts anti-leukemia activity by inhibiting cell proliferation through Gfi1 targeting



Acute myeloid leukemia (AML) is the most common malignancy of the bone marrow with a high mortality. Recent advances in high-throughput sequencing have led to the identification of various miRNAs implicated in the pathogenesis of AML. We found in this study that miR-650, a miRNA that was traditionally considered to participate in the onset of hepatocellular carcinoma, might play a significant role in AML development and progression.


qRT-PCR was used to detect the expression of miR-650 and Gfi1 in AML patients and healthy controls. Next, a luciferase assay was conducted to verify the target effect of miR-650 on Gfi1. Moreover, the CCK-8 assay was performed to evaluate the effect of miR-650 on the proliferation of AML cells in the presence and absence of Gfi1.


miR-650 was downregulated in AML whereas Gfi1 was upregulated. miR-650 could negatively regulate Gfi1 via direct targeting of its 3’-UTR, which was confirmed by luciferase assay. In addition, overexpression of miR-650 reduced cell proliferation in K562 cells, whereas an increase in cell proliferation was observed when K562 cells were transfected with miR-650 inhibitor, which was compromised in response to the knockdown of Gfi1.


Our research demonstrated that miR-650 modulates cell proliferation in AML through affecting the expression of Gfi1, which occurs by direct target action.

Post author correction




Changyong Yuan, Liming Xu, Pengcheng Du, Jinling Pang

Article History


Financial support: None.
Conflict of interest: None.

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  •  Department of Hemopathology, Dezhou People’s Hospital, Dezhou - China
  •  Pharmaceutical Preparation Section, Dezhou People’s Hospital, Dezhou - China
  •  Endoscopy Center, Dezhou People’s Hospital, Dezhou - China

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